Protein Engineering and Design

Author: Paul R. Carey
Publisher: Elsevier
ISBN: 9780080539973
Format: PDF
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The design and production of novel peptides and proteins occupy pivotal positions in science and technology and will continue to do so in the 21st century. Protein Engineering and Design outlines the rapid advances in computer-based modeling, protein engineering, and methods needed for protein and peptide preparation and characterization. This indispensable reference lays the groundwork for understanding this multidisciplinary activity while providing an introduction for researchers and students to the field of protein design. Introduces and defines the techniques involved in protein engineering and design Provides a concise overview of key technologies involved and demonstrates their contributions to the specialized design and production of novel proteins and peptides

Protein Engineering and Design

Author: Sheldon J. Park
Publisher: CRC Press
ISBN: 9781420076592
Format: PDF, Mobi
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Experimental protein engineering and computational protein design are broad but complementary strategies for developing proteins with altered or novel structural properties and biological functions. By describing cutting-edge advances in both of these fields, Protein Engineering and Design aims to cultivate a synergistic approach to protein science. Experimental Protein Engineering The first half of the book discusses experimental approaches to protein engineering and starts by describing several high-throughput screening platforms for protein engineering. Key techniques used for diversity generation are also discussed. The next few chapters present examples of therapeutics, enzymes, biomaterials, and other proteins that have been engineered by rational or combinatorial approaches. The section finishes with a chapter on the use of non-natural amino acids in protein engineering. Computational Protein Design The second half of the book introduces computational protein design, beginning with a chapter on computational and informatics algorithms used in protein engineering. Core components of computational protein design are then discussed in detail, and examples of heuristic protein design are provided. Subsequent chapters present examples of how computational design has played a critical role in advancing the field of protein engineering. Concluding with a chapter outlining current challenges in the field, this book makes computational protein design and diversity-oriented protein engineering widely accessible to a broad audience in academia and industry alike.

Protein Engineering

Author: Mallorie N. Sheehan
Publisher: Nova Science Pub Incorporated
ISBN: 9781626188785
Format: PDF, ePub, Docs
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Protein engineering is the process of developing useful or valuable proteins. It is a young discipline, with much research currently taking place into the understanding of protein folding and protein recognition for protein design principles. There are two general strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes. The second strategy is known as directed evolution and this is where random mutagenesis is applied to a protein, and a selection regime is used to pick out variants that have the desired qualities. This new book presents and reviews important data on protein engineering, such as application of engineered proteins and cell adhesive surfaces as scaffolds or other biomedical devices which has the potential to promote tissue repair and regeneration for a wide variety of tissues including bone and skin.

Protein Engineering Handbook

Author: Stefan Lutz
Publisher: John Wiley & Sons
ISBN: 3527666982
Format: PDF, ePub
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This introduction collects 17 innovative approaches to engineer novel and improved proteins for diverse applications in biotechnology, chemistry, bioanalytics and medicine. As such, key developments covered in this reference and handbook include de novo enzyme design, cofactor design and metalloenzymes, extremophile proteins, and chemically resistant proteins for industrial processes. The editors integrate academic innovations and industrial applications so as to arrive at a balanced view of this multi-faceted topic. Throughout, the content is chosen to complement and extend the previously published two-volume handbook by the same editors, resulting in a superb overview of this burgeoning field.

Protein Engineering Protocols

Author: Kristian M. Müller
Publisher: Springer Science & Business Media
ISBN: 1597451878
Format: PDF, ePub, Docs
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Protein Engineering Protocols considers the general, but not mutually exclusive, strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes. The second strategy is known as directed evolution. This volume provides a comprehensive guide to the methods used at every stage of the engineering process. It combines a thorough theoretical foundation with detailed protocols and will be invaluable to all research workers in the area, from graduate students to senior investigators.

Protein Engineering and Design

Author: Anton Torres
Publisher:
ISBN: 9781682864029
Format: PDF
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Protein Engineering is a nascent yet rapidly expanding field of study concerned with the useful development of proteins. This book elucidates new techniques and their applications in a multidisciplinary approach. The discipline of protein engineering is a growing field of study. This book traces the progress of this field and highlights some of its key concepts and applications. This field of study is understood through two major strategies that is - rational protein design and the directed evolution. The text has combined both these strategies so as to give it a holistic approach. Those in search of information on this field to further their knowledge will be greatly assisted by this book. It includes contributions of experts and scientists from across the globe which will provide innovative insights into this field.

Protein Engineering and Design with Non Canonical Amino Acids

Author:
Publisher:
ISBN:
Format: PDF, Docs
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The translation machinery of cells can be exploited for the incorporation of various non canonical amino acids into proteins. In this work, different fluoro-, amino-, and methyl- containing Tryptophan and Leucine analogues were incorporated into model proteins by the use of selective pressure incorporation (SPI) method. Such isosteric replacements introduced minimal local geometry changes in indole moieties, often at the level of single atomic exchange (atomic mutation) and normally do not affect 3D structures of substituted proteins, but induce significant changes in spectral and folding properties. For example, mutants of Barstar can be stabilised by incorporation of fluorinated Tryptophan analogues, while Tryptophan-fluorescence can be abolished by 4- and 7-Fluorotryptophan. Proteins substituted by toxic analogues can be specifically intracellulary delivered and after their recycling and the release of an analogue target, cells would be directed to apoptosis or necrosis.

Protein Design

Author: Raphael Guerois
Publisher: Springer Science & Business Media
ISBN: 1597451169
Format: PDF, Docs
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Recent major advances in our understanding of modulating protein functions has led to the development of new methods and algorithms to predict and decipher how amino acid sequences shape three-dimensional structures. Protein Design: Methods and Applications presents the most up-to-date protein design and engineering strategies so that readers can undertake their own projects with a maximum chance of success. The authors present integrated computational approaches that require various degrees of computational complexity, and the major accomplishments that have been achieved in the design and structural characterization of helical peptides and proteins. Other topics of discussion include: design of structural elementary motifs, entire proteins, and interfaces of protein complexes, and of amyloidogenic polypeptides and amyloid inhibitors. Authoritative and cutting-edge, Protein Design: Methods and Applications will be of major interest to protein scientists, biochemists, and all experimentalists selecting the strategy most adaptable for their design problem.